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THE DEVELOPMENT OF FUNCTIONAL CELL STRAINS
COLUMBIA UNIV NEW YORK
Three different cell culture devices have been developed which makes it possible to simulate in vivo conditions of cell growth under rigidly controlled conditions. The cytogenerator is a perfused suspension system in which the cell population is retained wholly within the growth chamber. The chemostat is a devise in which the population of logarithmically replicating cells is held constant by harvesting the cells at their replication rate. The pachcytogenerator is a device for growing solid masses of cells in an artificial stroma in vitro to more nearly simulate in vivo conditions of cell growth. Using these devices, an extensive analysis of the population dynamics and transition states of cells cultivated in vitro was completed. It was demonstrated that glycolysis is a transitory defense mechanism against excess glucose influx. Animal cells were found to react to virus infections by synthesizing a substance which inhibits viral replication. The immediate and long range plans for further research in cell culture are briefly outlined.
Annual progress rept., 1 Jul 1963-30 Jun 1964
Approved for public release; distribution is unlimited. Document partially illegible.