Acoustic methods have been used to detect and analyze periodic pressure fluctuations in a weakly ionized neon glow discharge. A pressure sensitive condenser microphone was used as a plasma probe. Comparison of acoustic pressures with the light intensity fluctuations measured with a photomultiplier tube have been made in an attempt to investigate a possible relationship between pressure fluctuations and moving striations. Neutrally charged particles have been shown to be the mechanism of the pressure fluctuations. Identical frequency measurements of the pressure fluctuations and moving striations, and a correlation between their intensities, indicate a relationship between the two phenomena.