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Accession Number:
AD0450950
Title:
REDUCTASE ACTIVITY AND SULFHYDRYLS IN THE DEHYDROGENASE HISTOCHEMISTRY OF HUMAN GINGIVA,
Corporate Author:
SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TX
Report Date:
1964-09-01
Abstract:
The nonenzymatic reduction of Nitro BT was studied in human gingival tissue sections at a pH of 7.5 and incubation times of 30, 60, 90, and 120 minutes in mixtures of Nitro BT and NAD with and without the addition of respiratory inhibitors. The presence of cyanide but not Amytal or azide resulted in timewise increases in Nitro BT reduction. The reduction of disulfide to sulfhydryl groups by cyanide under histochemical conditions was demonstrated by the reduction of Nitro BT which occurred after blocking of tissue sulfhydryl groups by pretreatment with n-ethyl maleimide. The results emphasize the importance of disulfide as well as sulfhydryl groups in tetrazolium dehydrogenase histochemistry. Author
Pages:
0007
File Size:
0.00MB
