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MECHANISM OF ENZYME ACTION
POLYTECHNIC INST OF BROOKLYN N Y
The research herein described has been an investigation of the mechanism of catalysis of proteolytic enzymes in general, and of alphachymotrypsin specifically. This was undertaken both by degradative studies of alpha-chymotrypsin itself and by the synthesis and kinetic investigation of possible model systems, both polymeric and of low molecular weight. An interesting effect recently discovered was the increase of catalytic rate constant or catalytic activity with increasing amount of imidazole in the polymers, independent of imidazole-group concentration in the solution, and no matter what the other groups in the polymer. Explanations for this phenomenon are being sought. The synthesis of imidazole-containing monomers, polymers, and low molecular weight model compounds, and the kinetic investigation of these species as catalysts, in the search for model systems of the proteolytic enzymes and the mechanisms of their action, continue. Author
Final rept. 1 Jan 59-31 Aug 61