Holsopples hypothesis J. Comp. Psychol., 4185-93, 1924 states that transfer of habituation is to be expected because repeated rotations in one direction yield equal amounts of practice for clockwise and counterclockwise accelerations irrespective of the rotation direction. To validate this observation, 3 groups I, II, and III of 10 subjects were given identical tests of postrotational oculogyral illusion OGI for clockwise and counterclockwise rotation before and after 34 clockwise trails in a Link trainer modified to rotate solely about its vertical axis. The rotation-rest ratios were 61.5 sec5 sec for I, 6.560 for II, and 91.590 for III. The conditions utilized in the experiment involved varying the indoctrination trials, rotational direction, and habituation series. Holsopples original hypothesis was not supported by the test results which suggest the following Habituation to rotation may occur when the effects of positive and negative angular accelerations overlap, but this habituation is not revealed by test trials administered before or after the habituation series, provided that these trials are stronger vestibular stimuli than those used in the habituation series. Holsopples hypothesis does not predict an equal reduction in the practiced and unpracticed rotation directions, regardless of the rotation-rest ratio employed. Holsopples generalization that habituation is dependent upon the time over which the response may act should be verified. Mowrers investigation of rotation-rest ratios with pigeons Comp. Psychol. Monograph, 91-48, 1934 should be repeated, and the habituation series as well as the trials should be recorded.