Photo-Oxidation From Mode-Locked Laser Exposure to hTERT-RPE1 Cells
NORTHROP GRUMMAN CORP SAN ANTONIO TX INFORMATION TECHNOLOGY
Pagination or Media Count:
Human retinal pigment epithelial RPE cells hTERT-RPE1 were used to detect photo-oxidation products generated from chronic NIR 810 nm laser exposure. Exposure of a discrete area within cell monolayers provided a means of distinguishing fluorescence above background levels. Oxidative stress was detected using the fluorescent dye H2DCF-DA and its analog CM-H2DCF-DA. Fluorescence was detected in cells exposed to mode-locked 76 MHz, -160 femtoseconds but not CW laser exposure. Detection of photo- oxidation from the mode-locked laser was dependent upon radiant exposure, but only if irradiance was greater than a threshold value. The CM-H2DCF-DA dye proved a more sensitive indicator of oxidation than H2DCF-DA, and the radiant exposure threshold for detection was dependent upon dye concentration. No oxidation was detected from CW exposures using the most sensitive fluorescent dye conditions when using 3 times the irradiance, and 10 times the radiant exposure needed to detect fluorescence from mode- locked exposure.
- Stress Physiology
- Lasers and Masers