Accession Number:

ADP019886

Title:

Lanthanide Enhanced Luminescence (LEL) with One and Two Photon Excitation of Quantum Dyes(copyright) Lanthanide(III)-Macrocycles

Descriptive Note:

Conference paper

Corporate Author:

VIRGINIA COMMONWEALTH UNIV RICHMOND DEPT OF CHEMISTRY

Report Date:

2004-01-01

Pagination or Media Count:

13.0

Abstract:

Improvements in the lanthanide enhanced luminescence LEL protocol have facilitated the use of the recently synthesized EuIII-macrocycle-mono-isothiocyanate, Quantum DyeRegistered, as a label. It was discovered that a homogeneous solution in ethanol or other solvent could be used to produce the lanthanide enhanced luminescence LEL effect, provided that the solution was permitted to evaporate. This protocol has been applied to the direct staining of cells in S phase, and was optimized for solid phase assays with Quantum Dye labeled streptavidin. Preliminary studies indicate that cells stained with the europium Quantum Dye can be observed both by conventional UV laser excitation and by infrared two-photon confocal microscopy. An enhancer has been found that enables the observation of simultaneous emissions from both the europium and terbium Quantum Dyes.

Subject Categories:

  • Inorganic Chemistry
  • Optics

Distribution Statement:

APPROVED FOR PUBLIC RELEASE