Accession Number:

ADP019730

Title:

Microfluidic Cell Volume Biosensor for High Throughput Drug Screening

Descriptive Note:

Conference paper

Corporate Author:

STATE UNIV OF NEW YORK AT BUFFALO DEPT OF MECHANICAL AND AEROSPACE ENGINEERING

Report Date:

2005-01-01

Pagination or Media Count:

6.0

Abstract:

The maintenance of cell volume is critical to health. Cell volume change reflects many biological and physicological processes. We have developed a lab-chip to measure cell volume change in real-time with high sensitivity and resolution, and applicable to both adherent and suspended cell populations. The volume change was detected by measuring the impedance of extra-cellular solution within a microfluidic chamber containing the cells. Using microfabrication to make precise chamber dimensions, volume change can be detected in response to an osmotic gradient 1mOsm. The sensor provides rapid screening of pharmaceutical agents affecting cell volume. We have screened for peptides that affect cell volume regulation and found one in spider venom that inhibits at 100pM.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research
  • Miscellaneous Detection and Detectors

Distribution Statement:

APPROVED FOR PUBLIC RELEASE