Accession Number:

ADP011225

Title:

Applications of Two-Photon Fluorescence Microscopy in Deep Tissue Imaging

Descriptive Note:

Corporate Author:

MASSACHUSETTS INST OF TECH CAMBRIDGE DEPT OF MECHANICAL ENGINEERING

Report Date:

2000-07-01

Pagination or Media Count:

10.0

Abstract:

Based on non-linear excitation of fluorescence molecules, two-photon fluorescence microscopy has become a significant new too for biological imaging. The point-like excitation characteristic of this technique enhances image quality by the virtual elimination of off-focal fluorescence. Furthermore, sample photodamage is greatly reduced because fluorescence excitation is limited to the focal region. For deep tissue imaging, two-photon microscopy has the additional benefit in the greatly improved imaging depth penetration. Since the near-infrared laser sources used in two-photon microscopy scatter less than their UVglue-green counterparts, in-depth imaging of highly scattering specimen can be greatly improved. In this work, we will present data characterizing both the imaging characteristics point-spread- functions and tissue samples skin images using this novel technology. In particular, we will demonstrate how blind deconvolution can be used further improve two-photon image quality and how this technique can be used to study mechanisms of chemically-enhanced, transdermal drug delivery.

Subject Categories:

  • Medicine and Medical Research
  • Optics

Distribution Statement:

APPROVED FOR PUBLIC RELEASE