Site-Specific Attachment of gold Nanoparticles to DNA Templates
OAK RIDGE NATIONAL LAB TN
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DNA was used as a scaffold for the binding of gold nanoparticles using a standard chemical technique. A DNA template was designed with amino-modified thymines located every 3.7 nm which would allow the attachment of the carboxylic acid functionalized gold nanoparticles. The gold particles were covalently bound to the amino groups on the DNA using standard 1-ethyl-3- 3-dimethylaminopropyl carbodiimide hydrochloride EDC chemistry in the presence of a competitor to block excess gold binding sites. The products were analyzed by transmission electron microscopy TEM and atomic force microscopy AFM.
- Industrial Chemistry and Chemical Processing