New Methods for Evaluating Skin Injury from Sulfur Mustard in the Hairless Guinea Pig
ARMY MEDICAL RESEARCH INST OF CHEMICAL DEFENSE ABERDEEN PROVING GROUND MD
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We have reported Proceedings of the 1991 Medical Defense Bioscience Review the use of a reflectance color meter for the quantification of erythema and a high frequency ultrasound system for the evaluation of edema by measuring skin thickness. This report compares these two techniques with several new methods including laser Doppler flowmetry LDF to measure microcirculation, skin elasticity measurements made both parallel Dermal Torque Meter, DTM and perpendicular Cutometer to the skin surface, and capacitance measurements for evaluating the skin hydration state. The skin of anesthetized hairless guinea pigs was exposed to saturated HD vapor 1.4mgmi at 4 sites for 3, 5, 7, or 9 minutes. Lesions were evaluated by each of the analytical methods at various times up to 24 hours post-exposure. Results have demonstrated a dose-response relationship between HD vapor exposure and erythema reflectance color meter, edema high frequency ultrasound, cutaneous microcirculation LDF, and skin elasticity measured perpendicular to the skin surface Cutometer. HD vapor cutaneous exposure had only a marginal effect on skin elasticity parallel DTM to the skin and on moisture content. These preliminary results suggest that reflectance color meter, high frequency ultrasound, LDF, and skin elasticity measurements may be useful for determining the efficacy of candidate antivesicant therapies.
- Chemical, Biological and Radiological Warfare