Comparative Metabolism, Cytotoxicity, and Genotoxicity of Chemical Carcinogens in Primary Cultures of Hepatocytes
CALIFORNIA UNIV DAVIS DEPT OF ENVIRONMENTAL TOXICOLOGY
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A major research objective of toxicologists is to develop bioassays that are rapid, inexpensive, sensitive, and predictive of potency and mechanism- of-action in humans. The routine production of primary cultures of hepatocytes of high viability is technically demanding, but is being successfully done in several laboratories. The recent development of the biopsy perfusion technique has expanded application to human liver, enabling studies that will provide data to better predict human susceptibility to chemical carcinogens. Considerable work remains to be done in developing a completely defined culture medium that will best maintain hepatocytes in their fully differentiated state. Although primary hepatocyte cultures provide a good cellular model of hepatocytes in vivo, they are often a poor model of the intact organism. Considerable caution must be used in interpreting data generated from primary hepatocyte cultures.
- Anatomy and Physiology