Accession Number:

ADP005149

Title:

Toxicity Monitored with a Correlated Set of Cell Culture Assays,

Descriptive Note:

Corporate Author:

ROCKEFELLER UNIV NEW YORK

Personal Author(s):

Report Date:

1985-05-01

Pagination or Media Count:

10.0

Abstract:

A set of assays have been developed in which cell cultures serve as an alternative to in vivo toxicity testing. One test uses a morphological screening procedure based on the highest dose of toxic agent tolerated HTD and is followed by a quantitative neutral red spectrophotometric absorption assay NR-90. The method has been standardized to make use of an automated microtiter plate reader. A second test is based on 50 percent inhibition of tritium uridine uptake UI-50 after incubation of the cultures with the toxicant. There is good agreement between these assays in the rank correlation of a broad spectrum of agents tested, as well as with the in vivo Draize rabbit eye irritancy data.

Subject Categories:

Distribution Statement:

APPROVED FOR PUBLIC RELEASE