Toxicity Monitored with a Correlated Set of Cell Culture Assays,
ROCKEFELLER UNIV NEW YORK
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A set of assays have been developed in which cell cultures serve as an alternative to in vivo toxicity testing. One test uses a morphological screening procedure based on the highest dose of toxic agent tolerated HTD and is followed by a quantitative neutral red spectrophotometric absorption assay NR-90. The method has been standardized to make use of an automated microtiter plate reader. A second test is based on 50 percent inhibition of tritium uridine uptake UI-50 after incubation of the cultures with the toxicant. There is good agreement between these assays in the rank correlation of a broad spectrum of agents tested, as well as with the in vivo Draize rabbit eye irritancy data.