Automated Epifluorescence Microscopy for Detection of Bacterial Contamination in Platelets
Patent Application, Filed 30 Jul 2001
DEPARTMENT OF THE NAVY WASHINGTON DC
Pagination or Media Count:
A method for determining the presence of bacteria in a platelet or red blood cell containing sample is disclosed. The method of the present invention includes the steps of lysing a substantial portion of the platelets or red blood cells staining the bacteria using a membrane permeable nucleic acid stain filtering the sample using a membrane filter with a suitable pore size so that a material containing the stained bacteria is retained on the membrane filter and analyzing the material retained on the membrane filter using epifluorescence microscopy andor digital image acquisition and analysis to determine the presence of bacteria in the sample. The method of the present invention allows the detection of bacterial contamination in platelets or red blood cells at clinically significant levels in a relatively short period of time.
- Medicine and Medical Research