Accession Number:

ADD019311

Title:

Bacteria Expressing Metallothionein Gene into the Periplasmic Space, and Method of Using Such Bacteria in Environment Cleanup.

Descriptive Note:

Patent, FIled 22 Nov 96, Patented 20 Oct 98

Corporate Author:

OFFICE OF THE SECRETARY OF THE NAVY WASHINGTON DC

Report Date:

1998-10-20

Pagination or Media Count:

14.0

Abstract:

A gene coding for the expression of metallothionein is incorporated into an expression vehicle for expression the metallothionein gene into the periplasmic space of a bacterium including the expression vehicle. It may also be possible to incorporate multiple copies of the metallothionein gene within a single bacterium. One typical expression vehicle is a p-MalP plasmid constructed to include the metallothionein gene. Using this expression vehicle, one can induce bacteria, such as transformed E. coli, to express metallothionein into the periplasmic space as a fusion product with, for example, the maltose-binding protein. Killed cell that have expressed metallothionein into the periplasmic space may then be attached to a solid support. The resulting device may be used to remove heavy metals from aqueous media.

Subject Categories:

  • Microbiology
  • Water Pollution and Control

Distribution Statement:

APPROVED FOR PUBLIC RELEASE