Bacteria Expressing Metallothionein Gene into the Periplasmic Space, and Method of Using Such Bacteria in Environment Cleanup.
Patent, FIled 22 Nov 96, Patented 20 Oct 98
OFFICE OF THE SECRETARY OF THE NAVY WASHINGTON DC
Pagination or Media Count:
A gene coding for the expression of metallothionein is incorporated into an expression vehicle for expression the metallothionein gene into the periplasmic space of a bacterium including the expression vehicle. It may also be possible to incorporate multiple copies of the metallothionein gene within a single bacterium. One typical expression vehicle is a p-MalP plasmid constructed to include the metallothionein gene. Using this expression vehicle, one can induce bacteria, such as transformed E. coli, to express metallothionein into the periplasmic space as a fusion product with, for example, the maltose-binding protein. Killed cell that have expressed metallothionein into the periplasmic space may then be attached to a solid support. The resulting device may be used to remove heavy metals from aqueous media.
- Water Pollution and Control