Surface Modification of Polymers with Self-Assembled Monolayers that Promote Adhesion, Outgrowth and Differentiation of Biological Cells
Patent, Filed 28 Apr 94, patented 24 Feb 98
DEPARTMENT OF THE NAVY WASHINGTON DC
Pagination or Media Count:
A process for forming spatially oriented neo-vascular capillaries comprising a providing a combination ultra-thin film UTF pattern of at least one cell adhesion promoter and at least one cell adhesion inhibitor wherein the cell adhesion promoters nave a linewidth of between about 50-490 microns b seeding the combination UTF pattern with endothelial cells at an initial seeding cell density c adding a first medium for incubating the seeded endothelial cells until the endothelial cells are grown to confluency d replacing the first medium with a second medium at confluency and e allowing the endothelial cells to differentiate into spatially oriented neo-vascular capillaries.
- Anatomy and Physiology
- Adhesives, Seals and Binders