Accession Number:

ADD018402

Title:

In Vitro Amplification/Expansion of CD34+ Stem and Progenitor Cells.

Descriptive Note:

Patent, Filed 28 Oct 93, patented 4 Feb 97,

Corporate Author:

DEPARTMENT OF THE NAVY WASHINGTON DC

Report Date:

1997-02-04

Pagination or Media Count:

24.0

Abstract:

The present invention relates to a method of amplifying in vitro stemcells. In this method hematopoietic CD34 stem and progenitor cells are isolated from human bone marrow and contacted with endothelial cells. The contacted stem cells and endothelial cells are cultured in the presence of at least one cytokine in an amount sufficient to support amplificationexpansion of the hematopoietic CD34 stem and progenitor cells. This method produces increased yields of hematopoietic CD34 stem and progenitor cells which can be used in human therapeutics.

Subject Categories:

  • Information Science
  • Biochemistry
  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE