Membrane Based Dot Immunoassay and Method of Use.
Patent, Filed 30 Dec 91, patented 6 Apr 93,
DEPARTMENT OF THE NAVY WASHINGTON DC
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Antigens or antibodies are detected using a novel membrane based immunoassay. Known antigens or antibodies which will form complexes with antigensantibodies to be assayed are spot filtered with pressure through a membrane. The membrane, either by itself or attached to a base material as a test strip, is incubated with a test fluid. Consequently, the resulting antibody-antigen complex is incubated directly or after in intermediate anti-antibody incubation with enzyme conjugated immunoglobulin and exposed to substrate which produces a colored insoluble product if the test target is present.
- Medicine and Medical Research