Method of Making Lipid Tubules by a Cooling Process.
Patent, Filed 29 Feb 88, patented 5 Feb 91,
DEPARTMENT OF THE NAVY WASHINGTON DC
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Diacetylenic phosophocholines have distinctly different endothermic and exothermic transition temperatures. Lipid tubules are formed by hydrating a diacetylenic phosphocholine at a temperature above its endothermic transition temperature. The hydrated lipid is then cooled slowly to a formation temperature 1 deg to 10 deg C. below the exothermic transition temperature to form tubule structures. The tubules structures can be polymerized to form permanent tubules. The tubules can be used in the same manner as a liposome vesicle or they can be metal coated for a variety of applications. It is therefore an object of the improved process of this invention to produce tubular structure from a broad variety of diacetylenic phospholipids. It is another object of the present invention to provide a method of producing tubules with a low amorphous material contamination. It is a further object of this invention to produce tubules of a relatively uniform length for each lipid variety from a broad variety of diacetylenic is to produce tubules in good yield for any diacetylenic phospholipid.