Accession Number:

ADD014651

Title:

Microbiological Assay Using Bioluminescent Organism.

Descriptive Note:

Patent, Filed 21 Dec 87, patented 21 Aug 90,

Corporate Author:

DEPARTMENT OF THE NAVY WASHINGTON DC

Personal Author(s):

• Stiffey, Arthur V.

Report Date:

1990-08-21

Pagination or Media Count:

5.0

Abstract:

A microbiological assay based on bioluminesce employing the bioluminescent dinoflagellate Pyrocystis lunula. An oil well drilling fluid sample is prepared according to E.P.A. procedures to obtain a suspended particulate phase sample. An aliquot of the sample is added to a growth medium containing Pyrocystis lunula in suspension. The mixture is agitated to subject th Pyrocystis lunula to a shear stress. Light emitted as a result of the shear stress on the Pyrocystic lunula is measure and compared with a control to determine if there is diminution of light produced by the Pyrocystis lunula in the mixture. Diminution of light production is an indication of the presence of a toxic substance in the sample. Patents. JS

Descriptors:

• *BIOLUMINESCENCE
• BIOASSAY
• DINOFLAGELLATA
• DRILLING
• FLUIDS
• GROWTH(GENERAL)
• LIGHT
• MICROBIOLOGY
• OIL WELLS
• PATENTS
• PRODUCTION
• SHEAR STRESSES
• TOXICITY

Subject Categories:

• Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE