Microbiological Assay Using Bioluminescent Organism.
Patent, Filed 21 Dec 87, patented 21 Aug 90,
DEPARTMENT OF THE NAVY WASHINGTON DC
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A microbiological assay based on bioluminesce employing the bioluminescent dinoflagellate Pyrocystis lunula. An oil well drilling fluid sample is prepared according to E.P.A. procedures to obtain a suspended particulate phase sample. An aliquot of the sample is added to a growth medium containing Pyrocystis lunula in suspension. The mixture is agitated to subject th Pyrocystis lunula to a shear stress. Light emitted as a result of the shear stress on the Pyrocystic lunula is measure and compared with a control to determine if there is diminution of light produced by the Pyrocystis lunula in the mixture. Diminution of light production is an indication of the presence of a toxic substance in the sample. Patents. JS