Method of Producing a Plaque Dispersing Enzyme.
Filed 9 Feb 81, patented 4 May 82,
DEPARTMENT OF THE NAVY WASHINGTON DC
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The present invention relates to an enzyme useful as an oral therapeutic agent and more particularly to a method of producing alpha-1, 3-glucan 3-glucanohydrolase which can be used to attack glucans of dental plaque. Dental plaque is a deposit which accumulates on the teeth and adjacent surfaces in the oral cavity. The plaque is a product of microbial growth resulting from food residues in the mouth. Mucoproteins and minerals present from the saliva and dead cells in the mouth also assist in plaque formation. A method of producing alpha-1, 3-glucanase by introducing a bacterial culture such as Pseudomonas sp. isolate NRRL B-12324 into an aqueous medium containing a limit glucan substrate which is greater than 90 percent alpha-1, 3-glycosidically linked, then allowing growth to take place to accumulate a-1, 3-glucanase, and then recovering the enzyme for use as an oral therapeutic agent.
- Medicine and Medical Research