Sensitive Radioimmunoassay Using Antibody to L-Hyoscyamine.
DEPARTMENT OF THE ARMY WASHINGTON DC
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A sensitive and specific radioimmunoassay is developed for L-hyoscyamine resulting from atrophine administration with which a concentration as low as 25 pgml using 0.1 ml of sample can be measured without the need for extraction. Specificity studies indicate that an antibody according to this invention has high specific recognition of L-hyoscyamine with only about 37 cross reaction with the D-hyoscyamine enantiomer of atropine. The antibody is produced from an immunogen having conjugated at least 42 and preferably 45 L-hyoscyamine-p-aminobenzoic acid haptenic molecules per molecule of bovine serum albumin. An antibody according to this invention can be used with a dilution titer as high as 12000.