Monoclonal Antibodies to Cholesterol and Methods.
DEPARTMENT OF THE ARMY WASHINGTON DC
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This patent application relates to monoclonal antibodies which demonstrate specific reactivity to cholesterol and methods for the detection of high levels of cholesterol by contacting biological specimens containing cholesterol with the monoclonal antibodies and measuring the formation of antigen-antibody complexes by immunosorbent assay. Monoclonal antibodies which demonstrate specific reactivity to cholesterol are provided. The antibody is characterized by its ability to induce complement-dependent immune damage to high-cholesterol liposomes which contain dimyristoyl phosphatidyl choline and high cholesterol, but not to low-cholesterol liposomes containing dimyristoyl phosphatidyl choline and low cholesterol. Cholesterol antigens which may be characterized as reactive with the monoclonal antibodies of the invention are provided. Also provided are compositions comprising a hybrid continuous cell line that produces antibodies having specific reactivity to cholesterol which comprises a cell hybrid of an animal spleen immunized with liposomes containing dimyristoyl phosphatidyl choline, cholesterol and lipid A fused to a myeloma derived from the same animal species as the spleen cell and a culture medium for the hybrid.