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Flow Cytometric Measurement of Total DNA and Incorporated Halodeoxyuridine.
DEPARTMENT OF THE AIR FORCE WASHINGTON DC
Pagination or Media Count:
A method for the simultaneous flow cytometric measurement of the total DNA content and the level of DNA synthesis in normal and malignant cells is disclosed. The sensitivity of the method allows a study of cell cycle traverse rates for large scale cell populations as well as single cell measurements. A DNA stain such a propidium iodide is used as the probe for the measurement of total DNA content and a monoclonal antibody reactive with a DNA precursor such as bromodeoxyuridine BrdU is used as a probe for the measurement of BrdU uptake by the cells as a measure of DNA synthesis.
APPROVED FOR PUBLIC RELEASE