Study of the Regulation of Telomere Replication by Characterizing the Cdc-13p Pathway in Yeast
Annual summary rept. 1 Jan 1999-31 Dec 2001
PRINCETON UNIV NJ
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Telomeres are repeated double stranded DNA sequences at chromosome ends. Replication of telomeres requires telomerase. Recent data suggest telomere length maintenance might be involved in the origin or progression of cancer and aging. Understanding of telomere replication could offer new targets for drug screen. The conservation of telomerase and telomere structure has made yeast a good system to study the problem. An essential yeast telomere binding protein Cdc13p appears to play a key regulatory role in telomere replication. This study showed that Pol alpha is involved in telomere replication. Cdc13p regulates telomere length during telomere replication by interacting with both telomerase and Pol alpha. This study also identified Cdc13p and yeast PCNA interaction. This interaction might beimportant for limiting C-strand degradation, process necessary for telomere replication and can kill cells when not limited. Other Cdc13p interacting proteins were also identified. Further studies are in progress.
- Medicine and Medical Research