Regulation of C-myc Gene Expression by Potassium Channel Blocker Quinidine in MCF-7 Human Breast Cancer Cell Line
Annual summary rept. 1 Jul 2000-30 Jun 2001
WEST VIRGINIA UNIV RESEARCH CORP MORGANTOWN*
Pagination or Media Count:
C-myc is a protooncogene whose gene product has a regulatory role in cell cycle progression, cellular differentiation, and apoptosis. The abnormal expression of c-myc was reported in 32 of breast cancers, suggesting its importance in the genesis andor progression of breast cancer. Therefore, it is important to understand the precise molecular mechanisms of c-myc regulation. The goal of this proposal is to elucidate how potassium channel blocking agent, quinidine, regulates expression of c-myc gene in breast cancer cells. Specific aim 1 was to test effects of quinidine on c-myc promoter activity completed, identify quinidine responsive element QRE within c-myc promoter completed, and study effects of quinidine on proteinsDNA interactions in the QRE in progress. The aim 2 was to test effects of quinidine on c-myc transcription rate by nuclear run-on assay. In the current report the PI proposed to substitute this aim with the one that tests effects of quinidine on c-myc mRNA stability, due to the technical difficulties to detect small changes in c-myc transcription rate by run-on assay not initiated. The significance of this proposal is that its results will improve our understanding of c-myc gene regulation and might lead to discovery of new targets for breast cancer therapy.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research