Visual Servoing for Optimization of Anticancer Drug Uptake in Human Breast Cancer Cells
Annual rept. 1 Sep 1999-31 Aug 2000
CALIFORNIA UNIV BERKELEY
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The purpose of this two-year IDEA proposal is to gather preliminary data that demonstrate the feasibility of a new class of in vitro drug response and chemosensitivity assays. Our objectives are to develop an instrument a visual servoing optical microscope, or VSOM in a stepwise fashion and gather preliminary data to aid future development of cell culture techniques and fluorescence assays. Our goal is to demonstrate the feasibility of using VSOM technology to analyze the heterogeneity and physiological characteristics of tumor cells from individual breast cancer patient biopsies. We believe that the VSOM will be extremely useful in developing new VSOM chemosensitivity fluorescence assays for a variety of breast cancer drugs. At the conclusion of this grant, we will have preliminary data that demonstrate the use of a VSOM doxorubicin chemosensitivity assay based on the dynamic physiological responses of single cells.
- Medicine and Medical Research