Role of Human DNA Polymerase and Its Accessory Proteins in Breast Cancer
NEW YORK MEDICAL COLL VALHALLA
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We have utilized polymerase 8 immunoaffinity chromatography and immunoprecipitation studies to link DNA replication to the cell cycle. Understanding the cell cycle leads to greater understanding of cancer. Tracking down new cell cycle genes and studying their function in the cell cycle will help to increase our understanding of this basic biological process at both the molecular and genetic levels. Another hypothesis that underlies the proposed studies during this period is that defects in DNA polymerase 8 and its accessory proteins contribute to the molecular etiology of sporadic and hereditary breast tumors. Normal and breast cancer cell lines and tissues were screened for genetic alterations in the POLD 1 gene. The search for changes were focused on target sequences which could give rise to changes in the fidelity of DNA polymerase 8. The target sequences were the 3, to 5 exonuclease regions of polymerase 8. Mutations that are found will be introduced into the recombinant proteins which will be expressed, isolated and characterized to establish their molecular phenotypes.
- Genetic Engineering and Molecular Biology
- Anatomy and Physiology