The Cellular Targets of Estrogen in Mammary Ductal Development
Annual rept. 1 Jun 1999-31 May 2000
CALIFORNIA UNIV SAN FRANCISCO
Pagination or Media Count:
It is known that the actions of estrogen in mammary development are mediated primarily by the estrogen receptor alpha, but it is not known which estrogen receptors, those in stroma or those in epithelium mediate mammary development. Our purpose is to probe this question by constructing transgenic mice in which wild type human estrogen receptor hER and mutants of the receptor that are super- active either at the classical ERE or alternative AP-i pathway are selectively expressed in epithelium or stroma. A further purpose is to explore the importance of the AP-1 versus the classical pathway in estrogen effects on ductal development. In the first year of this project we have constructed vectors for expression of wild type and mutant human estrogen receptors in the mammary epithelium of transgenic mice, and have made a pilot study of the effects of expressing these receptors in the reproductive epithelium of transgenic mice. The mice transgenic for a human ER super-active at AP-1 show gross abnormalities and hyperproliferation of the reproductive tract.
- Genetic Engineering and Molecular Biology
- Anatomy and Physiology
- Medicine and Medical Research