Genetic and Dynamic Analyses of Murine Peak Bone Density
Annual rept. 27 Sep 1998-26 Sep 1999
JACKSON LAB BAR HARBOR ME
Pagination or Media Count:
During our third year, the major effort has continued with genetic analyses of normal bone mineral density using inbred strains of mice differing by 35-50 in volumetric BMD. All C57BUL6x C3HHeF2 and C57BL6x CASTEiF2 mice have been phenotyped for femoral vBMD and 36 x C3HF2 have been phenotyped for vertebral vBMD. Both F2 populations have been genotyped for the 10 of mice with the highest and 10 with the lowest bone densities. Genome-wide analyses of B6x CASTF2 mice revealed strong vBMD loci on 4 Chromosomes Chrs 1, 5, 13, 15 and suggestive vBMD loci on 4 additional Chrs 3,4, 10, 14. Genome wide analyses of B6x C3HF2 mice revealed strong vBMD loci on 5 Chrs 1,4, 11, 13, 18 and suggestive vBMD 1oci on 3 additional Chrs 6, 14, 16. Congenic strains carrying Chr regions from CAST 1, 3, 5, 13, 14 or regions from C3H 1, 4, 6, 13, 18 have significantly altered femoral vBMD as a consequence of the donated Chr segment. Preliminary mapping data for B6x C3HF2 vertebrae reveal 4 vBMD loci shared with femurs 1,4, 14, 18 and 2 unique loci Chr 7 9. Lastly, a new DEXA instrument for mice, the PIXImus, has been validated for body composition, and demonstrated capable of detecting the major loci for BMD shared with the femoral bone site.
- Anatomy and Physiology
- Medicine and Medical Research