Role of Seprase in Breast Cancer Invasion
Annual rept. 1 Sep 1997-31 Aug 1998
ARKANSAS UNIV AT LITTLE ROCK
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This report documents progress made in the second year of DAMDI7-96-1-6097 Role of seprase in breast cancer invasion. The goals are to clone a full length cDNA encoding seprase and then produce cells that overexpress seprase to investigate its role in tumor cell invasion. This year an apparent full-length seprase cDNA has been amplified by RT-PCR from MDA-MB-436 human breast cancer cells. Moreover, 0.78 and 1.5 kb partial chicken embryo seprase cDNAs have been cloned and characterized. Peptides based on the deduced C-terminal 341 amino acids of chicken embryo seprase have been produced and used to immunize mice. An enzyme linked immunosorbent assay ELISA has been established for seprase-specific peptides. This ELISA will be used to screen hybridoma supernatants to identify hybridomas that produce seprase-specific antibodies. Two papers and a submitted manuscript describing new results and interpretations of various aspects of this project were completed this year. One of these papers was presented at the DoD Breast Cancer Research Program meeting Era of Hope. The research progress closely approximates the time line proposed in the original statement of work, thus we anticipate successful completion of the proposed research within the 4 year project period.
- Anatomy and Physiology
- Medicine and Medical Research