Accession Number:

ADB249668

Title:

Chromatid Paints: A New Method for Detecting Tumor-Specific Chromosomal Inversions

Descriptive Note:

Annual rept. 30 Sep 1997-29 Sep 1998

Corporate Author:

LOS ALAMOS NATIONAL LAB NM

Personal Author(s):

Report Date:

1998-10-01

Pagination or Media Count:

16.0

Abstract:

The goal of this project is to develop a new tool for karyotype analysis called chromatid painting. With fluorescence microscopy, chromatid painting of inversions produces a distinctive pattern, a switch in labeling from one chromatid to the other within the inverted region. This easily recognized pattern will permit rapid identification of previously unidentified tumor-specific inversions in BrCa tumor cells, thus improving diagnostic and prognostic capabilities. Significant progress has been made towards achieving Technical Objective 1 of our proposal. All of the steps required to prepare a chromatid paint have been shown to be feasible, although some require further optimization. We have demonstrated that 1 chromosomal DNA is not damaged during preparation procedures in a manner that would render it unsuitable as a template for DNA synthesis, 2 PCR amplification can be performed starting with very small quantities of template, 3 chromosomal DNA can be cloned by our methods, and 4 probes can be prepared from cloned and amplified DNA. As a byproduct of these efforts, two new and useful cytogenetic techniques were devised. An alternative approach to chromatid painting has been identified and will be examined more carefully in the future.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE