Chromatid Paints: A New Method for Detecting Tumor-Specific Chromosomal Inversions
Annual rept. 30 Sep 1997-29 Sep 1998
LOS ALAMOS NATIONAL LAB NM
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The goal of this project is to develop a new tool for karyotype analysis called chromatid painting. With fluorescence microscopy, chromatid painting of inversions produces a distinctive pattern, a switch in labeling from one chromatid to the other within the inverted region. This easily recognized pattern will permit rapid identification of previously unidentified tumor-specific inversions in BrCa tumor cells, thus improving diagnostic and prognostic capabilities. Significant progress has been made towards achieving Technical Objective 1 of our proposal. All of the steps required to prepare a chromatid paint have been shown to be feasible, although some require further optimization. We have demonstrated that 1 chromosomal DNA is not damaged during preparation procedures in a manner that would render it unsuitable as a template for DNA synthesis, 2 PCR amplification can be performed starting with very small quantities of template, 3 chromosomal DNA can be cloned by our methods, and 4 probes can be prepared from cloned and amplified DNA. As a byproduct of these efforts, two new and useful cytogenetic techniques were devised. An alternative approach to chromatid painting has been identified and will be examined more carefully in the future.
- Anatomy and Physiology
- Medicine and Medical Research