DID YOU KNOW? DTIC has over 3.5 million final reports on DoD funded research, development, test, and evaluation activities available to our registered users. Click
HERE to register or log in.
Accession Number:
ADB239319
Title:
Detection of DNA Adducts in Human Breast Tissue
Descriptive Note:
Annual rept. 1 Jul 97-30 Jun 98
Corporate Author:
NORTHEASTERN UNIV BOSTON MA
Report Date:
1998-07-01
Pagination or Media Count:
23.0
Abstract:
The purpose of this project is to optimize and apply new methodology for the detection of unknown DNA adducts in breast and, for comparison, other human tissues. In this methodology the DNA adducts are labeled with a fluorescent dye to enhance their detection. Although the first dye tested IMI1 was not fully stable, this problem was overcome by preparing IMI2. High dye Purity is required, and this was accomplished for IMI2 by means of flash chromatographysubmarine gel electrophoresis, which brought the purity to 99.9998. A basic protocol has been set up for the entire method, starting with human cell culture or human autopsy tissue samples, including breast samples and finishing with detection by capillary electrophoresis with laser induced fluorescence detection CE-LIF. Standard adducts, after labeling with IMI2, were detected by matrix assisted laser desorption ionization mass spectrometry MALDI-MS, with 300 fmol in the laser beam. Thus, the methodology is emerging as planned.
Distribution Statement:
APPROVED FOR PUBLIC RELEASE
