Accession Number:

ADB238008

Title:

Characteristics of Uterine Derived Growth Inhibitor (UDGI): A Novel Growth Inhibitor of Estrogen Receptor Negative Breast Cancer Cells

Descriptive Note:

Annual rept. 19 May 1997-18 May 1998

Corporate Author:

MCGILL UNIV MONTREAL (QUEBEC) LADY DAVIS INST FOR MEDICAL RESEARCH

Personal Author(s):

• Huynh, Hung

Report Date:

1998-06-01

Pagination or Media Count:

22.0

Abstract:

In our previous work we examined the molecular basis for the association of estradiol and tamoxifen with endometrial carcinoma. Our results showed the inhibitory effects of uterine fluid on the growth of breast cancer cells. Crude uterine derived growth inhibitor UDGI activity was reversible and non-toxic in the mechanisms of action. The present work was conducted to characterize, identify, and purify the proteins primarily responsible for the described UDGI activity and to characterize its biological activity in vitro. The 21-22 kDa UDGI protein was purified to near homogeneity and we are in the process of analyzing the UDGI amino-terminal sequence. To further define UDGI, antibodies against it and the cloning of cDNA encoding the UDGI protein are required to determine its primary structure and to assess likely biological actions. In addition, the characterization of UDGI expression patterns and regulatory pathways are needed to define molecular regulatory mechanisms and to assess its possible functions in breast epithelial cell growth and differentiation.

Descriptors:

• *INHIBITORS
• *ESTROGENS
• *BREAST CANCER
• EPITHELIUM
• HORMONES
• PROTEINS
• NEOPLASMS
• DEOXYRIBONUCLEIC ACIDS
• IN VITRO ANALYSIS
• ANTIBODIES
• CLONES
• GENETIC ENGINEERING
• CELLS(BIOLOGY)
• MAMMARY GLANDS
• GROWTH(PHYSIOLOGY)
• UTERUS

Subject Categories:

• Biochemistry
• Genetic Engineering and Molecular Biology
• Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE