Accession Number:

ADB233747

Title:

A New Insulin-Like Growth Factor Binding Protein (mac 25) and Its Role in Breast Cancer and Cell Growth Control

Descriptive Note:

Annual rept. 19 Aug 1996-18 Aug 1997

Corporate Author:

WASHINGTON UNIV SEATTLE

Report Date:

1997-09-01

Pagination or Media Count:

29.0

Abstract:

Mac25 is the seventh member of the IGFBP family IGFBP-7. IGFBP-7 is expressed in normal human mammary epithelial cells HMECs and upregulated in senescent HMECs. IGFBP-7 is not expressed in twelve of sixteen breast cancer cell lines, in studies by two laboratories. Its expression is absent in estrogen receptor positive ER breast cancer cell lines. IGFBP-7 may regulate HMEC growth. We will test the hypothesis that it suppresses growth in ER breast cancer cells. We will also test the ability of IGFBP-7 to negatively affect growth rates in ER breast cancer cells by transducing the cDNA into normal and human breast tumor cultures using a retroviral vector system. We will determine if IGFBP-7 is regulated at the transcriptional level by characterization of the promoter region of the gene. We will determine binding properties of IGFBP-7 protein by gel shift analysis of protein extracts and anti-IGFBP7 antibody. We will determine if IGFBP7 is hormonally regulated by studying tissue specificity of RNA expression and protein localization through in situ hybridization and immunohistochemistry additional aim.

Subject Categories:

  • Biochemistry
  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE