Parasite Lactate Dehydrogenase for Diagnosis of Plasmodium Falciparum. Phase II.
Final rept. 24 Mar 95-23 Mar 97
FLOW INC PORTLAND OR
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We have developed diagnostic assays based on the specific detection of Plasmodium lactate dehydrogenase pLDH activity. The assays have been developed into two basic formats. The first format is an Immuno-Capture pLDH assay IcpLDH in which active enzyme is captured and purified from whole blood with an immobilized monoclonal antibody. Enzyme activity is then measured colorimetrically using special substrates specific for pluN. The second format is an immuno-chromatographic test which measures pLDH protein. The immuno-chromatographic test is in a dip-stick format and has trademarked as OptiMAL. The analytical sensitivity of both tests is between 0.001 and .0001 parasitemia. Furthermore, using the panel of monoclonal antibodies, this assay can not only detect but differentiate between p. falciparum and non p. falciparum malaria. We have conducted over field studies and found that beth assays had clinical sensitivity of 200 parasitesmicroliters. Most importantly, we find that with either the quantitative ICpDH assay or the qualitative OPtiMAL assay, pLDH levels were coincident with parasite levels determined by microscopy thus making it possible to predict the success of chemotherapy based on pLDH levels.