Accession Number:

ADB227185

Title:

Development of a Sensitive and Specific Antigen-Detection System for Strongyloides Stercoralis and Hookworm Infections

Descriptive Note:

Final rept. 8 Nov 1996-7 May 1997

Corporate Author:

INTEGRATED DIAGNOSTICS INC BALTIMORE MD

Personal Author(s):

Report Date:

1997-06-01

Pagination or Media Count:

38.0

Abstract:

Prototype antibody and antigen capture DS assays have been developed for the detection of S. stercoralis and human hookworms. Using commercial immunoreagents that were available during the time line of phase I, antibody capture DS manufactured in pilot production were able to differentiate helminth infected patients from uninfected patients, but were not able to differentiate between S. stercoralis and hookworm infected patients subsequent immunoblot characterizations demonstrated that the antigen extracts used as capture antigen contained a large number of cross-reacting components. Antigen capture DS tests developed during phase I for the analysis of fecal samples have a sensitivity of detection of 5.0 ngsml or less. Data developed during phase I has elucidated a number of parasite specific antigens which will be useful in the production of efficacious, species specific antigen and antibody DS tests.

Subject Categories:

  • Medicine and Medical Research
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE