Molecular Cloning and Function of FAS/APO1 Associated Protein in Breast Cancer.
Final rept. 9 May 94-8 May 96,
LA JOLLA CANCER RESEARCH FOUNDATION CA
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Cell-mediated immunity represents one of the chief natural defense mechanisms against tumors. Cytolytic T-cells have two principal mechanisms for killing neoplastic cells a secretion of perforins and granzymes and b expression of the cytotoxic cytokine Fas-ligand, also known as APO-1 and CD95, is a member of the Tumor Necrosis Factor TNF receptor family. Fas is widely expressed on the surface of various types of cells in the body and provides a conduit through which Cytolytic T-cells CTLs and Natural Killer NK cells can trigger apoptosis of the tumor target cells. The ability of Fas to mediated cell death in human tumors however varies widely, and is unrelated to the relative levels of surface expression of this receptor protein. Using the cytosolic domain of Fas as a bait for yeast two-hybrid screening of cDNA libraries, 2 cDNA clones were obtained from a screen of over 10 million clones which encoded proteins that specifically bound to Fas.
- Genetic Engineering and Molecular Biology
- Anatomy and Physiology
- Medicine and Medical Research