Accession Number:

ADB219168

Title:

Vasopressin and Breast Cancer

Descriptive Note:

Annual rept. 1 Sep 95-31 Aug 96

Corporate Author:

DARTMOUTH COLL HANOVER NH

Personal Author(s):

Report Date:

1996-09-01

Pagination or Media Count:

44.0

Abstract:

Research indicates that neuropeptide hormones vasopressin, oxytocin are produced by breast cancer cells. Benign fibrocystic breast disease however does not appear to express the vasopressin gene. Such results indicate that expression of neuropeptide genes may represent part of the carcinogenic process. The interaction of neuropeptide through autocrineparacrineendocrine mechanisms with receptors on breast cancer cells represents a way in which these peptides might influence cancer cell pathophysiology. However, the expression of neuropeptide receptors and the evoked signaling cascades in breast cancer cells have not been thoroughly examined. An RT-PCR approach, using primers designed to amplify the specific vasopressin receptors and oxytocin receptor, has been implemented. Using this approach, PCR products for VACM, Vib, and V2 vasopressin receptors, and the oxytocin receptor have been amplified from breast cancer cells. Vasopressin Via receptor expression was not demonstrated using RT-PCR. Using Northern blot and a probe against human VACM, 3 mRNAs 3.5,5,6.5Kb were detected. In MCF-7 cells, vasopressin and a V1 agonist induced tyrosine phosphorylation of MAPK. The results indicate that breast cancer cells express neuropeptide receptors and that activation of these receptors can stimulate signaling events associated with cancer cell growth.

Subject Categories:

  • Biochemistry
  • Genetic Engineering and Molecular Biology
  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE