Accession Number:

ADB216072

Title:

In Field Detection of Biologicals in Human Blood Serum, Saliva and Urine Using Pan Coated Quartz Crystals

Descriptive Note:

Final rept. 1 Mar-31 Aug 1996

Corporate Author:

UNIVERSAL SENSORS METAIRIE LA

Personal Author(s):

Report Date:

1996-10-01

Pagination or Media Count:

40.0

Abstract:

Quartz crystal microbalance biosensors to detect ricin in complex biological matrices such as serum, saliva and urine have been developed. The sensors utilize a novel polyacrylonitrile PAN or protein G coating which is insensitive to non-specific fouling. The biosensor relies on simple interaction of immobilized anti-ricin antibody against the free antigen in solution. As antigen attaches to immobilized antibody, a frequency change is detected due to the additional mass on the piezoelectric crystals surface. The sensors will detect nanogram per milliliter amounts of ricin from these matrices with little or no sample pre-treatment or dilution. The biosensor assay is fast with results possible in approximately 500 seconds. The use of biosensor-based assays, in-field, may expedite clinical analysis and triage for exposure to possible biological warfare agents.

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research
  • Coatings, Colorants and Finishes

Distribution Statement:

APPROVED FOR PUBLIC RELEASE