Accession Number:

ADB216066

Title:

Development of a Lightweight, Portable, Minimally-Invasive Sensor for the Multi-Site Determination and Quantitation of Physiologic Lactate. Phase 1

Descriptive Note:

Final rept. 15 Mar-14 Sep 1996

Corporate Author:

HELLER (E) AND CO ALAMEDA CA

Personal Author(s):

Report Date:

1996-10-01

Pagination or Media Count:

37.0

Abstract:

The feasibility of developing a portable device for immediate monitoring of physiologic lactate using Wired EnzyneTM technology was demonstrated. Ultimately, this device will provide critical data for monitoring and dictating the triage and therapy of wounded soldiers in the battlefield or injured civilian trauna patients. Recent publications have established the importance of monitoring lactate in assessing a trauma patients condition. Lactate monitoring in local veins is critical in treatment of trauma related injuries. Because hypoxia and shock can develop rapidly, frequent if not continuous monitoring of high risk patients is necessary. We designed and built a breadboard mini fluidics system with electronics support for automated calibration and sampling. Studies in phosphate buffer PBS , cerebral spinal fluid CSF and controlled serum CBS led to an optimization of the Wired EnzymeTM sensor. We completed the Phase I program by rapidly and accurately monitoring changing lactate levels, resulting from trauma, in rats whole blood. This confirmed the unique ability to rapidly and closely follow changes in lactate in circulating blood from normal to lethal levels. Response was checked by a commercial instrument. Results were practically independent of oxygen over a wide range of oxygen partial pressures.

Subject Categories:

  • Medicine and Medical Research
  • Organic Chemistry
  • Logistics, Military Facilities and Supplies

Distribution Statement:

APPROVED FOR PUBLIC RELEASE