Accession Number:

ADB205608

Title:

Rapid Assay of Cellular Immunity in Q Fever.

Descriptive Note:

Final rept. 15 Mar-14 Sep 95,

Corporate Author:

BIOTECHNOLOGY TRANSFER INC COLUMBIA MD

Personal Author(s):

Report Date:

1995-10-01

Pagination or Media Count:

36.0

Abstract:

Cell mediated immunity CMI is a critical part of the immunological response to many infectious, neoplastic, and autoimmune diseases. Standard methods of measuring CMI are time consuming and tedious and are often limited in sensitivity. In Phase I studies, a prototype for a simple, rapid, and sensitive assay for CMI was developed. This assay is based on immunomagnetic separation of CD4 lymphocytes followed by bioluminescent detection of ATP levels in the separated cells. The assay uses only a small sample of blood, is complete within 24 hours, and can be performed on a number of samples simultaneously with minimal hands-on time. The performance of the assay was tested using peripheral blood mononuclear cells stimulated for 24 hours with mitogens or antigens. T cell mitogens reproducibility showed signalbackground values of 10 while cells cultured with tetanus toxoid, a recall antigen, showed signalbackground values of 2-10. The assay was used to test peripheral blood mononuclear cells from mice immunized with Q fever antigens and specific responses were detected in samples obtained 7 days after the initial immunization. This technology may be valuable in development of vaccines for Q fever and other infectious diseases and for assessing immune status of individuals related to Q fever or other agents.

Subject Categories:

  • Medicine and Medical Research
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE