Accession Number:

ADB190882

Title:

Glycosphingolipids as Putative Receptors for Staphylococcal Enterotoxin-B in Human Kidney Proximal Tubular Cells.

Descriptive Note:

Final rept.,

Corporate Author:

JOHNS HOPKINS UNIV BALTIMORE MD

Personal Author(s):

Report Date:

1994-06-20

Pagination or Media Count:

32.0

Abstract:

Staphylococcal enterotoxin-B is an exotoxin capable of producing severe food poisoning in experimental animals and man. The proximal tubular PT cells in the kidneys of experimental animals are the major site of this toxin. We have found that 125I-SEB is specifically bound to a digalactosylceramide present in human kidney and cultured human kidney PT cells. In contrast, 125I-SEB binding to glycosphingolipids GSL derived from rat kidney, human brain, or human intestine was not observed. The absence of this receptor in rat kidney may suggest a possible biochemical basis of immunity to SEB in such animals. We also found that the biological responses of PT cells consistent to the binding of 125I-SEB and certain SEB-peptide domains are release of lactate dehydrogenase, cell death, and the production of nitric oxide. The latter concurs with pathophysiological observations of SEB-induced shock and the dilation of the blood vessels in the kidney in monkeys. Glycosphingolipids, Receptors, Kidney proximal tubular cells, High performance thin layer chromatography.

Subject Categories:

  • Microbiology
  • Anatomy and Physiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE