Accession Number:

ADB190603

Title:

Parasite Lactate Dehydrogenase for the Diagnosis of Plasmodium Falciparum.

Descriptive Note:

Final rept.,

Corporate Author:

FLOW INC PORTLAND OR

Personal Author(s):

Report Date:

1994-08-08

Pagination or Media Count:

16.0

Abstract:

We have developed a rapid and quantitative method for the detection of all species of malarial parasites that infect humans. The assay is based on a patented process that can specifically measure Plasmodium sp. Lactate dehydrogenase pLDH. The assay is reproducible and has been standardized with the use of recombinantly expressed PLDH. The sensitivity of the original patented process has been greatly enhanced with the use of dye ligands and antibodies that specifically recognize pLDH. These reagents have been used to rapidly capture the pLDH enzyme and facilitate separation of the enzyme from factors that contribute to background signal. Our current pLDH technology can be formatted as either a wet assay or a dry dip-stick test that can measure PLDH activity form either whole blood, Red Blood Cells, or serumplasma. This technology may also be applied to measure the drug-sensitivity of parasites isolated from individual patients. Our current technology, the pLDH test is now ready for verification in the field. Malaria, Plasmodium SP, Diagnosis, Lactate dehydrogenase, Culture and Sensitivity, Drug development, Drug resistance, Falciparum

Subject Categories:

  • Medicine and Medical Research
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE