Accession Number:

ADB169611

Title:

Production of Polyclonal Antibodies in Rabbits

Descriptive Note:

Final rept. 1 Mar-30 Sep 1992

Corporate Author:

BIOTEK INC WOBURN MA

Personal Author(s):

Report Date:

1992-09-30

Pagination or Media Count:

36.0

Abstract:

Cholinesterases are widely distributed throughout the body. They hydrolyze acetylcholine ACh, a compound which serves also as the neurohumoral agent in peripheral junction transmission. Cholinesterases are known to be crucial for cholinergic neurotransmission. The objective of the Phase 1 application is to raise antibodies from rabbits against cholinesterase, butyrylcholinesterase, glycosylated butyrylcholinesterase, synthetic peptides that mimic selected areas of the HIV virus proteins, and also against monoclonal antibodies which inhibit the catalytic activity of cholesterase. The immunogens used in the preparation of the antibodies will be supplied by WRAIR. During Phase I of the SBIR contract polyclonal antibodies were successfully raised from rabbits against acetylcholinesterase, butyrylcholinesterase, and five synthetic peptides using immunogens supplied by WRAIR. The binding capacity of the peptide antisera was determined by enzyme linked immunoassay. Most of the titers at half maximal absorbance were greater than 1 x 104. All antibodies were submitted to WRAIR. Antibodies against the enzymes were assayed by WRAIR for IgG and enzyme content.

Subject Categories:

  • Medicine and Medical Research
  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE