Development of New Immunogens and a Controlled Release Delivery System for Oral Immunization Against Staphylococcal Enterotoxin B
Annual draft rept. 15 Sep 1990-31 Dec 1991
SOUTHERN RESEARCH INST BIRMINGHAM AL
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During this reporting period, experiments were performed in which rhesus monkeys were immunized with an SEB toxoid microsphere vaccine delivery system. The immune response were followed and the monkeys ultimately received a challenge with lethal doses of aerosolized SEB toxin. The vaccine delivery system consisted of SEB toxoid microencapsulated in a 5050 polyDL-lactide-co- glycolide excipient. The immunization schedule consisted of the nine possible combinations of intramuscular IM, oral, and intratracheal IT primary and secondary immunizations. Primary immunizations were given on Day 0 and secondary immunizations were administered on Day 49. Two of the four planned replicate monkey experiments were concluded during this reporting period. The monkey antibody levels suggested that an IM primary followed by and IT or oral secondary immunization would provide the highest degree of protection. For the first time, the ability to protect monkeys against an aerosol challenge with a lethal dose of SEB toxin was documented. The monkeys that received an IM primary immunization followed by an IT secondary immunization survived.
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