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Accession Number:
ADB155812
Title:
Rapid Bioassay Monitoring System for Water Quality (Rapid Detection of Toxicants in Potable Waters by Laser Light Scattering)
Descriptive Note:
Final rept. 23 Apr 1984-15 Nov 1987
Corporate Author:
WYATT TECHNOLOGY CO SANTA BARBARA CA
Report Date:
1989-03-01
Pagination or Media Count:
64.0
Abstract:
A laser-based bioassay system has been developed for the rapid detection of a broad range of toxicants in potable waters. Exponential phase cultures of two members of an isogenic set of Eacillus subtilis are prepared from lyophiles. Water samples to be tested are prepared by filtration, pH factor adjustment, and chlorine neutralization. For each B. subtilis strain wild type WT 168 and mutant fh 2006-7 a ml water sample is prewarmed to 39 C and inoculated with 0.15 ml of the culture. Similar control samples are prepared with deionized, filtered water. Laser light scattering measurements of the four samples are made 6 minutes and 66 minutes following inoculation. From these recorded measurements, an on-line personal computer determines the presence or absence of toxicants. This report describes the instrumentation and protocols developed to achieve this rapid assay. The results of a detailed statistical analysis of key elements of the assay in the absence of non-toxic contaminants are presented using data from the Wyatt Technology Corporation laboratory and the Southern Research Institute. A set of 53 blind samples was subsequently processed and yielded an accuracy for detection in excess of 94.
Distribution Statement:
APPROVED FOR PUBLIC RELEASE
