Alphavirus Epitopes of Vaccine Relevance.
Final rept. 1 Jul 78-31 Aug 87,
MARYLAND UNIV BALTIMORE SCHOOL OF MEDICINE
Pagination or Media Count:
The original overall goal of studies supported initially by these contracts was to define the role of virus-specific effector T cells in mediating recovery from, and immunity to, alphavirus infections with a view to improving or redesigning alphavirus vaccines. These studies utilized an established animal model in which a neuroadapted variant naSIN of he AR339 strain of Sindbis virus SIN produced infections that were either paralytic and lethal or subclinical and self-limiting for C57BL6 B6 mice that had been inoculated, respectively, with naSIN intracerebrally i.c. and intraperitoneally i.p.. From immune animals in the latter category, pools of sera and splenic lymphocytes were assessed individually for their capacity to adoptively confer, to nonimmune syngeneic recipients, protection against a normally lethal i.c. naSIN challenge. In repeated experiments it was found that protection by immune lymphocyte transfer i.p. was minimal it occurred in a minority of recipients and only when the cells were administered at least 24 hours prior to virus challenge.