Crotoxin: Structural Studies, Mechanism of Action and Cloning of Its Gene
Final rept. 1 Mar 1986-30 Nov 1989
WYOMING UNIV LARAMIE DEPT OF MOLECULARBIOLOGY
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The purpose of this project is to 1 gain greater insight into crotoxin and crotoxin homolog structure, in order to provide a better understanding of this class of rattlesnake neurotoxin 2 develop as in vitro system for examining presynaptic neurotoxin mechanism of action 3 clone the crotoxin gene as a first step in creating a non-toxic, but immunoreactive crotoxin analog and 4 explore other possible non-toxic, crotoxin immunogens as potential vaccines against crotoxin and its homologos. Acidic ad basic subunits of crotoxin were sequenced and their higher-ordered structure examined by circular dichorism, fluorescence and FTIR. Effect of crotoxin subunit cross- linking on its toxicity and phospholipase activity were examined. Keywords Crotoxin, Presynaptic neurotoxins, Rattlesnake toxins, Natural toxins.
- Medicine and Medical Research