Accession Number:

ADB145621

Title:

Chemical Blistering: Cellular and Macromolecular Components

Descriptive Note:

Annual rept. 1 Jan 1988-31 Dec 1989

Corporate Author:

MICHIGAN UNIV ANN ARBOR

Personal Author(s):

Report Date:

1989-12-31

Pagination or Media Count:

36.0

Abstract:

The overall purpose of this investigation is to elucidate the molecular mechanisms by which bisbeta-chloroethyl sulfide BCES exerts its vesicant action when applied topically to human skin. The technical objectives of the project are to establish morphological, cytochemical, andor biochemical indicators of epidermal mustard toxicity and to examine and evaluate the relevance of these indicators to vesication. The following three biological systems have been used in this study a stratified, cornified primary cultures of keratinocytes grown on nylon microporous membranes positioned at the air- medium interface b submerged monolayer cultures of primary keratinocytes that contain early differentiated as well as germinating cells and are grown in a medium having a low level 0.08-0.10 micronmo of Ca2Calcium ions and c cultures of the BSC 40 line of monkey kidney cells grown as a submerged monolayer. Keywords Mustard, Keratinocyte, Tissue culture, Alkylation, Toxicity, Chemical blistering, Metabolism, DNA Repair, Mitochondria, Epidermal differentiation, RAV, BCES, BSC.

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE